Evolving reversible immunocapture by membrane sensing peptides: towards scalable extracellular vesicles isolation - MARVEL
Abstract: MARVEL mission is to combine and implement reversible capturing and peptide science, towards the first and best performing ever affinity-based technology for scalable small Extracellular Vesicles (EVs) isolation. The modularity in scaling-up of the novel protocols and kits will be demonstrated on medium/large sample volumes in relevant environments for therapeutic and diagnostics use of EVs.
MARVEL will introduce the use of membrane-sensing peptides (MSP) as novel ligands for the size-selective capturing of small EV, unbiased by differential surface protein expression (membrane as universal EV marker); in parallel, specific peptide probes (SPP) with high affinity for clinically relevant EV protein markers will be developed to enrich selective EV subpopulations. The versatility and modularity in scaling-up of the technology will be demonstrated in:
1) the manufacturing of GMP-compliant EV as a medicinal product for cardiac repair;
2) laboratory scale urine-based liquid biopsy
Activity : 1/11/2020 - 28/02/2023